![]() ![]() That's it! You're done! From Git Bash, do the following to test: ssh ls Now ssh into the destination server, then do mkdir -p ~/.ssh Scp ~/.ssh/id_rsa.pub the bit on your own computer done.
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![]() Streptavidin-coated beads bind and remove the small biotinylated fragment from the digestion. The amplified oligos are then digested at high-temperature to expose the microbead barcode as a single-stranded DNA overhang. Upon arrival, each sub-pool is PCR amplified from the pool with a biotinylated primer. After the oligo design is completed, the library is ordered from a commercial oligo pool vendor. Typically, the library is split into batches of 384 or 1536 genes, each with a unique pair of sub-pool amplification primers. All of the oligos needed to assemble one particular gene are given the same microbead barcode. Restriction sites and a microbead barcode are added to each oligo. Briefly, the genes to be synthesized are first bioinformatically split into several fragments, such that each fragment can fit on an oligo. We then break the emulsion and recover our library of assembled genes.Ī visual description of DropSynth can be seen in the video above. Then the barcodes are removed, and gene assembly takes place inside of those droplets by polymerase chain assembly (basically PCR). The beads are then emulsified, thereby isolating and concentrating the oligos into a picoliter-sized droplet. DropSynth overcomes this barrier by isolating individual assembly reactions in a vortexed emulsion, allowing for the entire gene library to be assembled in one pot.ĭropSynth uses a set of barcoded beads, such that each bead pulls down the required oligos for a particular gene’s assembly. Previous gene synthesis methods require isolating individual gene assemblies in different reactions, which becomes cost-prohibitive for assembling thousands of genes and requires expensive automation equipment. FAQ What is DropSynth?ĭropSynth is a new gene synthesis method whereby large gene libraries are assembled from microarray-derived oligo libraries within water-in-oil droplets. Multiplexed Gene Synthesis in Emulsions for Exploring Protein Functional Landscapes. Plesa C*, Sidore AM*, Lubock N, Zhang D, Kosuri S. DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions bioRxiv, DOI: 10.1101/740977 (2019) Sidore AM*, Plesa C*, Samson JA, Kosuri S. ![]() 2020 - Multiplexed Characterization of Protein Families with DropSynth The video below provides a summary of how the DropSynth process works.ĭropSynth Summary from Calin Plesa on Vimeo. This website provides resources for scientists who wish to use DropSynth to assemble their own gene libraries. DropSynth was invented in the Kosuri Lab at UCLA and is now being developed in the Plesa Lab at UOregon. DropSynth is a simple, low-cost method to build thousands of genes from microarray-derived oligos in a single reaction. ![]() Affirm is one of the few buy now, pay later providers that offer consumers long-term financing options with a 0% APR. It offers POS loans ranging from one to 60 months with a limit of $25,000 per loan. Who's this for? Affirm is a good choice if you need a longer-term financing option with a 0% APR and no late fees. Investing +More All Investing Best IRA Accounts Best Roth IRA Accounts Best Investing Apps Best Free Stock Trading Platforms Best Robo-Advisors Index Funds Mutual Funds ETFs Bonds Help for Low Credit Scores +More All Help for Low Credit Scores Best Credit Cards for Bad Credit Best Personal Loans for Bad Credit Best Debt Consolidation Loans for Bad Credit Personal Loans if You Don't Have Credit Best Credit Cards for Building Credit Personal Loans for 580 Credit Score Lower Personal Loans for 670 Credit Score or Lower Best Mortgages for Bad Credit Best Hardship Loans How to Boost Your Credit Score Taxes +More All Taxes Best Tax Software Best Tax Software for Small Businesses Tax Refunds Small Business +More All Small Business Best Small Business Savings Accounts Best Small Business Checking Accounts Best Credit Cards for Small Business Best Small Business Loans Best Tax Software for Small Business Personal Finance +More All Personal Finance Best Budgeting Apps Best Expense Tracker Apps Best Money Transfer Apps Best Resale Apps and Sites Buy Now Pay Later (BNPL) Apps Best Debt Relief Best Mortgages for Average Credit Score.Best Loans to Refinance Credit Card Debt. ![]() The take-home message here is that you should by all means use Tabatas on a regular basis in your training to maximize fat loss as well as cardiovascular conditioning. ![]() With Shortcut to Shred, this means three (or six) Tabatas per day, as all six workouts train three body parts: chest, triceps, and abs on Days 1 and 4 shoulders, legs, and calves on Days 2 and 5 and back, traps, and biceps on Days 3 and 6. This will make Shortcut to Shred a slightly different program, but if you've already done it before using cardioacceleration, a change of pace might be welcome.Īs with Shortcut to Size, you'll simply add one or two rounds of Tabatas after your last exercise of each muscle group. ![]() So if you're wanting to add Tabatas to the program, you'll probably want to omit the cardioacceleration rather than try and do both HIIT techniques. But you're not limited to these types of exercises.įirst of all, as written, Shortcut to Shred already includes cardioacceleration techniques that accomplish the same goal as Tabatas. These are all fine choices I program them often for Tabatas. Tabata intervals are typically done using standard cardio activities such as the stationary bike, mountain climbers, jumping jacks, or a rowing machine. First is what I just alluded to: It's nice knowing you don't have a cardio session waiting for you right after finishing a tough lifting workout-or ever! More than that, forcing your body to go back and forth between lifting weights and HIIT cardio promotes a type of muscle confusion-a "shock" to the system, if you will-that will keep your metabolism ramped up for maximal fat burning. I like intertwining lifting and HIIT cardio in this manner for a couple of reasons. No need to tack on any extra cardio, as you've already done a total of 12-24 minutes of HIIT cardio during the workout. This way, by the time you've completed your Tabatas after triceps, the workout is completely over. You'll do Tabatas after training each of these muscle groups for a total of three rounds. For example, Workout 1 (as well as Workout 4) hits chest, shoulders, and triceps. In SS8, one or two Tabata rounds (depending on the week) are performed after every muscle group in all workouts throughout the eight-week program. And second, by using Tabatas on "non-cardio" exercises. How so? First, by inserting Tabatas throughout a workout, not just at the end or on a separate day altogether. Another common practice is to do Tabatas in a completely separate, non-lifting workout.īoth of these methods work fine, but personally, I like to get a little more creative when programming Tabatas while still reaping the same, if not better, cardio and fat-loss benefits. The most straightforward way is to tack on Tabatas, using exercises such as a stationary bike or other cardio activity, to the end of a lifting workout. off), there are a number of ways to use it. What's the Best Way to Use Tabata for Fat Loss?Īs simple as the Tabata formula is (8 x 20 sec. That's right, just 4 minutes of cardio can lead to significant fat-burning results, provided you keep the intensity high in all eight 20-second rounds. In other words, 20 seconds "on" and 10 seconds "off," times eight, which takes exactly 4 minutes. Izumi Tabata, consists of eight rounds of 20-second high-intensity intervals of a given exercise, with 10-second rest periods between each. In case you're unfamiliar with the technique, one bout of Tabata, named after the legendary Japanese exercise scientist Dr. Incorporate the Tabata protocol intelligently into your training, and you may never go back to traditional cardio. And as HIIT routines go, none are more efficient for fat loss-and even enhancing cardiovascular conditioning-than Tabata rounds. If you need further information on how to secure your residency, including contact details for prefectures, you may find the residency in France webpages from these organisations useful: Check the France visa wizard to see which nationalities require a visa. Nationals of certain non-EU countries may need a visa before travel. They must travel to France and then submit a WARP application to the local prefecture as your family member. Find more information on who this applies to on the Living in Europe page. Your close family members continue to be able to join you and settle in France at any point in the future. Read the French government advice on how to appeal a residency decision (in French). If your application is refused, you will be notified about the appeal process. Contact your prefecture to find out the process for renewing your WARP locally. You must also renew your WARP free of charge when it expires. You can add your marital name as a “nom d’usage” if you want this to appear on your residence card in addition to your birth name. If you change your name following marriage or civil partnership, your administrative documents will stay in your birth name (“nom de famille/naissance”). If your commune has been renamed or restructured you do not need to report this change or request a new WARP. If your situation changes (address, marital status, etc.) or you lose your card, you must report these changes and request a new WARP, free of charge, on the French Government portal. You will need to provide evidence of why your application is late. If you have not yet applied, you can still apply for your WARP if you have reasonable grounds for missing the deadline. You can also email the Interior Ministry: Keep copies of any correspondence.Ĭontinue to check your email and spam folder, and respond promptly to requests to help prefectures finalise your application quickly. If you have applied for a WARP but have not yet had your appointment or received your card, you must keep your certificate of application (from your initial email confirmation) and contact your prefecture. Contact your prefecture if your child is under 18 and has been asked to present a WARP for work purposes or for other reasons. If you applied for your Withdrawal Agreement Residence Permit (WARP), known in French as a ‘carte/titre de séjour « accord de retrait du Royaume-Uni de l’Union Européenne »’, by 4 October 2021, your rights are protected by the Withdrawal Agreement, pending a decision on your application.Ĭhildren do not require a WARP, but must apply at their prefecture when they turn 18 years old. Visas and residency if you were living in France before 1 January 2021 Contact your Mairie (local townhall) for more information CCAS (Centres Communaux d’Action Sociale) available in each commune.France Services (find your local office using the interactive map).information on joining a family member with a European nationality or non-European nationality already settled in Franceįor assistance in accessing your rights as a resident in France, the following public information services are available (in French):.guidance for UK nationals living in France.Information on how to register for residency.You must tell the UK government offices that deal with your benefits, pension and tax if you are moving or retiring abroad.Ĭheck the entry requirements for France and read the French government’s: See our travel advice for France for up-to-date information on entry requirements, local laws and customs, safety and emergencies. You should also read our Living in Europe page for detailed guidance about citizens’ rights under the Withdrawal Agreement. You should read these in addition to the rest of the guidance in each section. ![]() Some parts of this guide only apply if you have been living in France since before 1 January 2021. If you were living in France before 1 January 2021 ![]() follow the British Embassy Paris on Facebook and Twitter.Read general guidance on moving or retiring abroad. The Foreign, Commonwealth & Development Office (FCDO) is not liable for any inaccuracies in this information. You should get definitive information from the French authorities. This information is provided as a guide only. Read about how our consulates in Paris, Bordeaux and Marseille can help. This guide sets out essential information for British citizens moving to or living in France. ![]() The card’s utility and ability to be used in any deck eventually led to it being banned for offering an unfair advantage. At a time when the TCG was far slower than it is today, Pot of Greed was one of the few cards that any deck could play that would instantly provide access to new cards from your deck with zero cost. The three-word effect of Pot of Greed may appear naively simple at first, but it’s this simplicity that makes it one of the most powerful and iconic banned Yu-Gi-Oh! cards in the game today. “Draw 2 cards.” Pot of Greed's simple card-drawing ability made it one of the best Yu-Gi-Oh! cards ever made, but also led to its ban from the TCG. Its legacy continues as players find new and inventive ways of cycling through their decks, all to recreate the satisfaction that comes from drawing all five pieces. Despite this, none are quite as memorable or satisfying as Exodia, which remains one of the few Yu-Gi-Oh! cards with a unique win condition not to be banned from competitive play. In the years since this card’s release, several similar monsters - such as Victory Dragon - have introduced new mechanics that allow players to win through means other than taking your opponent’s life points to zero. With such a unique win condition, players immediately tried to put together decks that would give them the highest chance of drawing these pieces as quickly as possible. One of the first Yu-Gi-Oh! cards to introduce alternative win conditions to the TCG, Exodia The Forbidden One allows players to instantly win the duel provided they draw all five pieces of Exodia. Win on your terms One of the most powerful Yu-Gi-Oh! cards to emerge in the TCG's history, Exodia grants an instant win to the player who collects all five pieces of the legendary monster. Perhaps it’s no surprise that, just like in the TV show, top duels in the game’s infancy often recreated the Dark Magician vs Blue-Eyes match-up. As the rarest card to pull in the original Legend of Blue-Eyes White Dragon booster pack, a first-edition copy can sell at auction for thousands of dollars.Įven away from its monetary value, Blue-Eyes White Dragon is still an intimidating beast to face in a duel. Like Dark Magician, an entire archetype has spawned around it with the express purpose of making this 3000 ATK boss monster easier to summon, with monsters such as The White Stone of Legend being made just to make this monster easier to search and summon to the field.īeyond this, however, Blue-Eyes White Dragon can boast its status as one of the most valuable Yu-Gi-Oh! cards released to date. It would be impossible to mention Dark Magician in a list of Yu-Gi-Oh!’s most iconic cards without mentioning the boss monster of Yugi’s rival, Seto Kaiba. Its popularity resulted in an array of support cards, such as Magician Navigation, being created in order to make it easier for fans to use this iconic card.ĭark Magician may not be the most competitive card in Yu-Gi-Oh! today, but it’s a showcase of how the popularity of a single card can allow it to persevere as a viable strategy in duels for over 20 years.Ī card that soars Blue-Eyes White Dragon isn't just one of the best Yu-Gi-Oh! cards - it's also worth a pretty penny in its original edition. While maybe not as powerful today as it once was, for fans who grew up on the original TV series and remember Yugi winning with his Dark Magician, some players still wish to feel the same rush that comes from taking their favourite card to victory. Where else could we start a list of the best Yu-Gi-Oh! cards than with a mention of Yugi Muto’s iconic boss monster? When people think of Yu-Gi-Oh!, they think of Dark Magician - and in the early days of the card game before Synchro or Link monsters, a game-winning strategy could be as simple as copying your favourite anime character. Here are just a few of the most iconic monsters, spells and traps from this most auspicious of trading card games.Ī magical introduction Dark Magician is one of the most iconic Yu-Gi-Oh! cards of all time thanks to its star role in Yugi Muto's deck. There are plenty of Yu-Gi-Oh! cards that fit this bill from throughout the history of this iconic franchise. ![]() It is a very stripped down Linux distro which is packaged with a WindowsXP look-alike Desktop manager. Mint typically appeals to Windows users trying to learn Linux. There are multiple Backup Solutions available on the NAS, but you don't sound very confident in Linux, so I recommend that you stick to Backup Station, as it is documented for exactly what you want to do, and because you have an entire Community here that can assist you with it. It is specifically designed for doing Backups to your QNAP NAS. I have never even heard of FreeFileSync so I can't comment on it. I'm afraid I dislike Mint, so I've never played with it for more than a few hours. You can do RTRR (Replication) or simple Backups, that manual section I referred you to provides instructions for both strategies. And FreeFileSync for Linux Mint 18 is a Folder Comparison and Synchronization Software that Creates and Manages Backup Copies of all your Important Files. I have no idea what FTP Server Mint uses. The Tutorial shows you Step-by-Step How to Install FreeFileSync in Mint 18 Sarah/Serena/Sonya/Sylvia 64-bit GNU/Linux. FileZilla is offered as both an FTP Server and as an FTP Client. As I opened the thread 'FreeFileSync 11.29: unacceptable TLS certificate in Linux Mint 21.1' and, in particular, in the FreeFileSync forum I wrote 'In few words, it seems the issue is only under Linux Mint 21.1 (Ubuntu 22.04), not even in Linux Mint 20.3 (Ubuntu 20.04 based).Otherwise I really dont if there is something at repository level. So far FreeFileSync do for me a great jog itś scans/sync in 2 minutes wath files are newer or need to deleted. ![]() With your link to Remote Replication section NAS to NAS and Rsync I not understand so good what I can do with it and how it will used with FTP Filezilla? But deleted files Linux Mint will not deleted automaticly the TS-412. Retep2001 wrote:Mmmm, please explane more in detail, I have found linux mint filezilla and with this prog I can acces de Qnap. ![]() You can use the Picnik tools to edit the image and save the changes back to the album. The Picnik window will open within the same Picasa Web Albums tab as an overlay. To use Picnik to edit one of the images stored in Picasa Web Albums, click on the Edit button to the right-above the image or click on the Edit menu button and click on Edit in Picnik from the list. So what are you waiting for? Head on over to your Picasa Web Albums account and start getting creative with Picnik! If you have any questions use the comments form below.ġ. Picnik, a recent acquisition by Google, allows users to edit pictures from Flickr, Picasa Web Albums, and other services within a browser window. Now you can edit all of the pictures saved in Picasa Web Albums using Google’s Picnik online photo editor. Yes, most definitely! For now, Pixlr Editor is my first choice for editing photos and creating images when I don’t have Photoshop around.If you use Picasa and Picasa Web Albums to share your pictures with family and friends, you are going to love the latest feature announced at the Google Photos blog. And also for anyone who just needs to make a few quick adjustments to an image that is intended to be published on Flickr, Picasa or Facebook. Who can Pixlr Editor be useful for?īasically for anyone who needs to edit images or photos, but who doesn’t have access to any more advanced image/photo editing tool. My secondary computer, a small HP Mini-Note 2140 netbook with a single-core 1,6 GHz CPU and 2 Gb of RAM, has worked great with Pixlr Editor for me. Without knowing for sure, I would guess that computer performance will not be a problem for anyone who has a fairly new computer. There is also an optional Pixlr image storage account and a quick image hosting service called imm.io available.Īll you need to use Pixlr is an internet connection (I’ve used it with a 1,6 Mbps 3G connection without any problem) and an up-to-date web browser, and you are ready to go. PXD) or sent directly to external services such as Facebook, Flickr or Picasa. When saving an image, the file can be downloaded to the local computer in several different formats (including Pixlr Editor’s own multi-layer format. PSD files created in Adobe Photoshop and edit them directly, a feature which I find to be really useful when working away from my office. Layers, shapes, airbrushes, scaling, gradients, color adjustments and a number of filters on top of that. It supports many of the most common features that are found in regular software editors. Pixlr is also free to use, with an impressive feature set for an online image editor. It is launched directly from the site through Java, so as long as you have Java installed there is no installation of any kind needed – Pixlr runs directly in the browser. It is basically a web application with an appearance that will look familiar to anyone who has used Adobe Photoshop, Paint Shop Pro or GIMP. ![]() One out of several image editing tools on, a quite advanced one. Pixlr Editor is an online service for creating, editing, opening and saving images and photographs in various formats. ![]() Out of many different solutions I have tried, there is one that stands out as an excellent and easy-to-use way: The Pixlr Editor. Most often I use Adobe Photoshop CS5 for both tasks, but there are also situations where I need a lighter alternative, for example when I am using another computer than my main work machine. And more specifically, how I edit the photos and which software I use. ![]() Photo editing without having any software…īeside questions about the templates and themes themselves, one of the most common questions I get is how I create the images in my designs. Keep in mind that these will be my own impressions of the software and services, and that the best way to find out if a program or service works well for you is to give it a try yourself. I created it since I often get questions about my opinion about different kinds of software and services, and rather than rewriting the answers multiple times I will publish short reviews and opinions based on my own experiences in this category. This is the new “reviews” category of my blog. |